Table 2.

Reaction solutions.

Digestion buffer	0.5 mM ZnCl2, 2 mM DTT a, 0.05% Tween 20 in 50 mM HEPES, pH 7.4
Substrate	200 µM SNAPtide®520 b prepared in sterile water for injection

^a DTT = Dithiothreitol.

^b List Biological Laboratories, Inc., supplied through Calbiochem, San Diego, CA, USA (Cat #567333).