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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 Jun;98(3):1320–1327. doi: 10.1128/jb.98.3.1320-1327.1969

Changes in the Plasma Membrane of Escherichia coli During Magnesium Starvation

Annelise Fiil a,1, Daniel Branton a
PMCID: PMC315329  PMID: 4892379

Abstract

The effect of Mg++ starvation on the structure of the Escherichia coli cell membrane was studied with the freeze-etch technique. Special attention was paid to changes within the plane of the membrane, which in normal exponentially growing cells has a netlike arrangement of particles 2 to 6 nm in diameter. During Mg++ starvation, a paracrystalline particle pattern appeared on the plasma membrane, and large areas devoid of particles were seen. Although these changes are reproducibly associated with Mg++ starvation of the bacteria, no decrease in the Mg++ content of the cell envelope per se was detected, even after 24 hr of Mg++ deprivation. The structural changes caused by Mg++ deprivation appeared to involve specific and permanent alterations in membrane development. The absence of other nutrients or divalent cations did not induce similar alterations.

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Selected References

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