Table 1.
Retrotransposition activity of marked SVAs in HeLa HA cells
| Passenger | pcDNA.L1-RP |
No driver |
|||
|---|---|---|---|---|---|
| n | Number of G418R | Activity | n | Number of G418R | |
| SVA.10 mneoI | 3 | 124.7 ± 17.8 | 54.2 | 2 | 1.0 ± 1.0 |
| SVA.10B mneoI | 3 | 7.3 ± 1.9 | 3.2 | 2 | 0.0 ± 0.0 |
| SVA.2 mneoI | 3 | 14.7 ± 3.5 | 6.4 | 2 | 0.5 ± 0.5 |
| SVA.SRE1 mneoI | 2 | 2.5 ± 1.5 | 1.1 | 2 | 0.0 ± 0.0 |
| 99 SVA.SRE1 mneoI | 2 | 7.0 ± 0.0 | 3.0 | 2 | 0.0 ± 0.0 |
| ORF1 mneoI-AA | 3 | 11.3 ± 2.0 | 4.9 | 2 | 0.0 ± 0.0 |
| EGFP mneoI | 3 | 2.3 ± 0.7 | 1.0 | 2 | 0.0 ± 0.0 |
Transfection of approximately 2 × 106 HeLa HA cells was carried out in T-75 flasks with 8 µg of the driver plasmid DNA and 4 µg of the passenger plasmid DNA. In the column labeled ‘No driver', only 4 µg of the passenger plasmid DNA was transfected. Following G418R selection, flasks were stained and colonies counted. The data are presented as the mean number of G418R colonies across replicates (n ± SEM). Activity is the number of G418R for each passenger divided by the number of EGFP mneoI G418R.