Table 1.
CaSR Consensus Peptide Sequences Enriched in Biopanning Experiments
| Sample used in biopanning experimentsa | CaSR consensus peptide sequencesb | Amino acid residues of CaSRc | Number of clones with consensus sequence (%) |
|---|---|---|---|
| APS1-2 | AQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWL | 26–71 | 9/20 (45) |
| TVSKALAEATLSFVAQNKIDSLNLDEFCNCSE | 103–133 | 3/20 (15) | |
| APS1-3 | HFGVAAKDQDLKSRPESVECIRYNFRGFRWL | 41–71 | 8/16 (50) |
| KALAEATLSFVAQNKIDSLNLDEFCN | 106–130 | 2/16 (13) | |
| APS1-4 | HFGVAAKDQDLKSRPESVECIRYNFRGFRWL | 41-71 | 5/20 (25) |
| FVAQNKIDSLNLD | 114–126 | 3/20 (15) | |
| APS1-5 | LGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFR | 34–69 | 3/20 (15) |
| APS1-6 | AQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWL | 26-71 | 9/20 (45) |
| SRLLSNKNQFKSFLRTIPNDEHQAT | 171–195 | 2/20 (10) | |
| APS1-7 | GDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFR | 30–69 | 8/20 (40) |
| TVSKALAEATLSFVAQNKIDSLNLDEFCNCSE | 103–133 | 3/20 (15) | |
| APS1-8 | No consensus peptide sequence defined | — | — |
| APS1-9 | QKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWL | 27–71 | 2/20 (10) |
| APS1-10 | AQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWL | 26–71 | 10/20 (50) |
| SRLLSNKNQFKSFLRTIPNDEHQAT | 171–195 | 3/20 (15) | |
| APS1-11 | QKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWL | 27–71 | 2/20 (10) |
| APS1-12 | No consensus peptide sequence defined | — | — |
| APS1-13 | HFGVAAKDQDLKSRPESVECIRYNFRGFRWL | 41–71 | 10/20 (50) |
| SRLLSNKNQFKSFLRTIPNDEHQAT | 171–195 | 3/20 (15) | |
| APS1-14 | HFGVAAKDQDLKSRPESVECIRYNFRGFRWL | 41–71 | 8/20 (40) |
| SRLLSNKNQFKSFLRTIPNDEHQAT | 171–195 | 2/20 (10) | |
| APS1-15 | QKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWL | 27–71 | 3/20 (15) |
| TVSKALAEATLSFVAQNKIDSLNLDEFCNCSE | 103–133 | 3/20 (15) | |
| AHH | HFGVAAKDQDLKSRPESVECIRYNFRGFRWL | 41–71 | 9/20 (45) |
| Anti-CaSR Abd | YGPDQRAQ | 20–27 | 10/20 (50) |
| Anti-CaSR mAbe | ADDDYGRPGIEKFREEAEERDICI | 214–237 | 10/20 (50) |
a
APS1 samples 2 to 15 are sera from APS1 patients; AHH sample is a serum sample from the single AHH patient.
b
Peptide sequences enriched by biopanning of the phage-display CaSR peptide library with APS1 and AHH patient sera and animal anti-CaSR antibodies.
c
Amino acid residues are numbered according to the CaSR peptide sequence with the ATG initiation codon as residue number 1.
d
Polyclonal CaSR antibody.
e
Monoclonal CaSR antibody.