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Phosphate restriction impairs rhBMP-2-induced ectopic bone formation. BFAs were performed in mice fed chow (A, C, E, G) or the PRD (B, D, F, H) after implantation of a rhBMP-2-impregnated Helistat sponge. Histology was evaluated 6 (A, B), 9 (C. D; high-power E&F), and 14 (G) days after implantation. Sections are representative of those obtained in five independent experiments for each time point.
