Table 2.
Control (WB) | Hindlimb suspension (HS) | ||||||
---|---|---|---|---|---|---|---|
Day | 0 | 21 | 35 | 0 | 21 | 35 | |
n | 5 | 5 | 5 | 10 | 9 | 8 | |
Osteocalcin, ng/mL | Avg. | 2.47 | 1.72 | 2.07 | 1.93 | 1.39 | 1.32* |
SD | 1.93 | 0.78 | 0.52 | 0.83 | 0.85 | 0.48 | |
TRAP5b, U/L | Avg. | 0.77 | 0.94 | 0.88 | 0.85 | 1.16** | 1.16** |
SD | 0.19 | 0.20 | 0.11 | 0.17 | 0.43 | 0.30 |
Serum TRACP5b (RatTRAP, SBA Sciences) and OCN (Rat Osteocalcin, BT-490, Biomedical Technologies) were measured by ELISA [absorbance measured at 450 nm (OCN) and 405 nm (TRACP5b)] in order to determine relative systemic expression of markers of bone resorption and formation, respectively.
p = .07 versus HS day 0.
p = .07 WB versus HS for that day.