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. 2009 Oct 17;25(5):1147–1156. doi: 10.1359/jbmr.091031

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Copyright © 2010 American Society for Bone and Mineral Research

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Fig. 4 — Quantification of YFP⁺ cell proliferation. Representative images of Flk1-H2B::YFP and the cell proliferation marker Ki67. Colocalization of Flk1-H2B::YFP (yellow) and Ki67 (red) was detected in BMP-2-treated and control tissues. Graphs show the total number of YFP⁺/Ki67⁺ cells in the images taken within the area divided by the number of images analyzed. The area fraction was measured for nine at day 2 and five at day 4 BMP and eight at day 2 and four at day 4 control different areas, and the average area fraction was calculated for control and BMP-treated tissues. The area fractions of YFP⁺/Ki67⁺ nuclei in the control and the BMP-treated tissues on day 2 were 7.32 ± 3.26 and 10.20 ± 6.95, respectively. The area fractions for day 4 were 6.97 ± 2.32 and 11.26 ± 2.58 in the control and the BMP-treated tissues. Based on the Student's t test, the p value for the day 2 data was .29 and that for the day 4 data was .035. Taken together, the data showed significant YFP⁺/Ki67⁺ population increases by day 4 after the BMP treatment, but on day 2 there were no significant differences in dividing YFP cell population between control and BMP-treated tissues.