Table 1.
Differential Scanning Calorimetry (DSC) and SDS-PAGE Analysis of Type I Collagen
DSC | SDS-PAGE | ||||||
---|---|---|---|---|---|---|---|
Sample | Genotype | α12α2, % | α12α2m, %a | α13, % | α13, % | Reactive Cys-SH | α2m–S–S–α2m, %a |
Mouse | GG | 100 | 0 | 0 | 0 | 0 | 0 |
Mouse neo+ | GT | 59 ± 5b | 17 ± 1b | 24 ± 5b | 23 ± 5 | Yes | 1 ± 0.3 |
Mouse neo+ | TT | 0 | 38 ± 1b | 62 ± 1b | 67 ± 5 | Yes | 9 ± 3 |
Mouse Neo– | GT | 51 ± 1b | 49 ± 1b | 0 | 0 | Yes | 1.5 ± 0.3 |
Human | GG | 100 | 0 | 0 | 0 | No | — |
Human | GT | 50 ± 10b | 50 ± 10b | 0 | 0 | Yes | — |
The superscript m identifies mutant chains.
Estimated deconvolution error.