Table 3.
Time course of genes differentially expressed in kidney between S and S.SHR(2) located on chromosome 2
| Number | Accession# | Gene Name | Fold Change
|
Description | ||
|---|---|---|---|---|---|---|
| Week 4 | Week 12 | Week 20 | ||||
| 1 | AI501417 | Mgst2? | +2.4 | EST | ||
| 2 | BE107853 | +5.4 | +6.4 | EST | ||
| 3 | BF415556 | +5.5 | +4.7 | EST | ||
| 4 | BG374101 | +3.6 | +2.7 | EST | ||
| 5 | AA859982 | +2.6 | +2.2 | EST | ||
| 6 | AW141832 | +2.1 | similar to RIKEN cDNA 2310050P13 (LOC310808) | |||
| 7 | BF551322 | +2.2 | similar to CG5805-PA (LOC365841) | |||
| 8 | BG379771 | +2.8 | EST | |||
| 9 | BF553729 | Wnt2b | +2.7 | EST | ||
| AI555464 | Wnt2b | +2.4 | EST | |||
| 10 | BE097574 | Kcnab1 | +2.4 | EST | ||
| 11 | AA819288 | +2.3 | retinoic acid receptor responder protein 1 | |||
| 12 | BF411765 | Celsr2 | +2.1 | cadherin EGF LAG seven-pass G-type receptor 2 | ||
| 13 | AI715140 | +2.0 | similar to histone protein Hist2 h3c1 (LOC310679) | |||
| 14 | BF284175 | +2.3 | +2.4 | +2.2 | similar to group XII-1 phospholipase A2 (LOC362039) | |
| 15 | BF396545 | Sfrp2* | +2.4 | secreted frizzled-related protein 2 | ||
| 16 | AI511405 | −13.2 | −6.3 | similar to hypothetical protein FLJ22693 (LOC310467) | ||
| 17 | BF522861 | −2.1 | −2.1 | |||
| 18 | BF388224 | −7.4 | −8.7 | |||
| 19 | AI454310 | −2.0 | −2.5 | EST | ||
| 20 | BF402235 | Sars1 | −2.1 | EST | ||
| 21 | AI172217 | −2.9 | similar to protein beta-galactosidase, alpha | |||
| 22 | U86635 | Gstm5 | −2.1 | glutathione S-transferase, mu 5 | ||
| 23 | NM_021584 | −2.0 | −2.8 | activity/neurotransmitter-induced early gene protein 4 | ||
| 24 | BF290410 | −2.2 | EST | |||
| 25 | BE120370 | −2.1 | EST | |||
| 26 | NM_024157 | Cfi | −4.0 | complement factor I | ||
| 27 | NM_012889 | Vcam1 | −2.1 | vascular cell adhesion molecule 1 | ||
| 28 | AF367210 | Il7 | −2.1 | interleukin 7 | ||
| 29 | NM_031120 | Ssr3 | −2.9 | TRAP-complex gamma subunit | ||
| 30 | U05675 | Fgb | −2.3 | fibrinogen, beta polypeptide | ||
| 31 | BI288681 | −7.3 | −6.7 | −3.8 | transcription activator of D-serine dehydratase? | |
| 32 | BE100973 | −5.8 | −8.7 | −8.4 | Rattus norvegicus transcribed sequences | |
| 33 | AI454769 | −4.3 | −4.8 | −3.9 | Rattus norvegicus transcribed sequences | |
| 34 | AI043891 | Cct3* | −2.8 | −2.8 | −2.0 | |
| 35 | AI232217 | −4.2 | −3.7 | −2.2 | Rattus norvegicus transcribed sequences | |
| 36 | NM_031154 | Gstm3 | −5.1 | −4.7 | −3.4 | glutathione S-transferase, mu 3(Yb3) |
| 37 | M28241 | Gstm1 | −3.2 | −3.8 | −3.6 | glutathione S-transferase, mu 1 |
A positive fold change means that the transcript was expressed higher in the S.SHR(2) congenic compared with the S. Conversely, a negative value means that the S transcript was expressed at a higher level than the congenic. Transcripts found to have a fold-change ±2.0 or greater are included here; see Supplemental Table S1 for a complete gene list. Statistical calculations were performed by 2 methods (see MATERIALS AND METHODS FOR MORE DETAIL): P value (FWER) and adjusted P value (FDR using Benjamani and Hochberg correction for multiple corrections). Fold change data shown are all significant at least P < 0.05. For exact P value see Supplemental Table S1.
Both of these genes map to the reduced quantitative trait locus (QTL) interval, differential expression was confirmed using quantitative real-time PCR. Cct3 was found to be differentially expressed at all 3 time points, with a fold-change ranging from −1.20 to −1.35 (at least P < 0.05). Sfrp2 was observed to be differentially expressed at week 4 (confirming the Affymetrix data) and also at week 12 and 20, with a fold-change ranging from +1.60 to +1.79 (at least P < 0.05).