Table 1. Acoustophoretic Cell Separation.† .
| PBPC sample | Target | Waste | |
| Cell counts ‡ | |||
| WBC (×103/µl) | 13.4 (7.2–23.3) | 6.1 (1.9–15) | 0.1 (0–0.4) |
| PLT (×103/µl) | 86.5 (16–314) | 8.0 (0–61) | 31.0 (0–128) |
| RBC (×106/µl)# | <0.1 | <0.1 | <0.1 |
| Lymph (×103/µl) | 3.1 (1.9–13.7) | 1.5 (0.2–9.1) | - |
| Cell numbers § | |||
| WBC (×105) | 13.4 (7.2–23.3) | 7.8 (2.4–18.7) | 0.2 (0–0.8) |
| PLT (×105) | 86.5 (16–314) | 10.2 (0–78) | 62.0 (0–256) |
| RBC (×108) | - | - | - |
| Lymph (×105) | 3.1 (1.9–13.7) | 1.9 (0.3–11.6) | - |
| Distribution of separated cells | |||
| WBC (%) | 97.8 (80–100) | 2.9 (0–22) | |
| PLT (%) | 12.5 (0–54.6) | 89.0 (56.7–100) | |
Abbreviations: WBC, white blood cells; PLT, platelets; RBC, red blood cells; Lymph, lymphocytes.
†
Data are presented as the median (range) of 10 samples.
‡
The PBPC samples were diluted 1∶5 before cell count.
#
The RBC count was below the detection limit of 0.1 (×106/µl) given in the instrument manual [44].
§
The addition of wash buffer to the acoustophoresis-chip and the difference in flow rate between the central outlet (20 µl/min) and the side oultlet (2×20 µl/min), gave a sample dilution so that the cells of 100 µl PBPC sample were distributed to 128 µl target sample (central outlet) and 200 µl waste sample (side outlet). Cell numbers were calculated accordingly.