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. 2011 Aug 9;6(8):e23023. doi: 10.1371/journal.pone.0023023

Figure 5. VSV-mediated inhibition of MICA surface expression occurs at an early post transcriptional level.

Figure 5

A, JTag-9 cells were transiently transfected with 5 µg MICA*008-GFP (MICA*008) or MICA*009-GFP (MICA*009) vector constructs. Two hr post transfection, the cells were either mock infected or infected with 0.001 MOI VSV10 for 19 hr. The cells were analyzed for surface MICA/B expression by flow cytometry. The dot-plots represent one out of three experiments. B, JTag-9 cells were transiently transfected as described in A. Two hr post transfection, the cells were either mock infected (-) or infected with 0.001 or 0.01 MOI VSVΔM51 for 19 hr. The cells were analyzed for MICA/B surface expression by flow cytometry. The bar graphs show mean±SD from three experiments.