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. 2011 Aug 9;6(8):e23102. doi: 10.1371/journal.pone.0023102

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Sanz et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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This plasmid includes the M. circinelloides leuA and the P. blakesleeanus carRA genes. It was employed in the transformation of the M. circinelloides strains MS7, MS8 and MS21. Digestion with SacI results in plasmid linearization (12-kb single band). Digestion with SalI gives rise to two fragments of 7.3 kb (including the leuA gene) and 4.7 kb (including the carRA gene).