Figure 2. Lab yeast strains, labeled with YFP, cannot grow at low sucrose concentrations.

Cells were sorted into microtiter wells, each containing 150 µl of medium, at the given cell density and sugar concentration and allowed to grow for 85 h without shaking at 30°C. (A) Typical fluorescent scan of a plate containing 4 mM glucose plus 4 mM fructose inoculated with the indicated number of cells. (B) The fraction of wells with growth using strain yJHK111 (W303 genetic background). Results shown are totals of three experiments; each experiment used one plate per sugar concentration. Error bars refer to 95% binomial confidence interval using the adjusted Wald method. See Figure S2 for results using a strain with the S288C background.