Figure 3. Antibacterial activity of asCATH1 and 2 against three species of bacteria - Y. ruckeri (A), V. anguillarum (B) and E. coli (C).

Bacteria were grown to mid-log phase then incubated for 18 h with varying concentrations of the Atlantic salmon cathelicidins. LL-37 was included as a control for bacterial killing and E. coli ATCC 25922 was included as a control bacterium. Absorbance was read at 600 nm and percentage growth was calculated by comparison to the no inhibition control lane in the assay. The MIC was defined as 50% inhibition, which is indicated by a dotted line on each plot. Data shown are the mean ± SE of triplicate wells.