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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Neurobiol Dis. 2011 Jun 24;44(1):63–72. doi: 10.1016/j.nbd.2011.06.004

Figure 5. DM-0.1 inhibits the expression of NOX2 and oxidative stress response in the spinal cord tissue of EAE-II animals.

Figure 5

Mice were immunized with 50 μg MOG/0.5 mg/ml M.t. and treated with saline or DM-0.1 starting from day 7 until the peak of EAE (19–20 d.p.i.). Lumbar spinal cords were collected after perfusion with PBS and analyzed for the expression and activity of NOX2. DM-0.1 treatment (n = 4) could significantly reduce the mRNA level of p47phox in the spinal cord tissue when compared to saline (n = 6) (A). The transcription level of the enzymatic subunit gp91phox was also decreased in the spinal cord tissue after DM-0.1 treatment (B). A non-significant reduction in NADPH oxidase-dependent superoxide production was found in the spinal cord homogenates of DM-0.1 treated animals (D). The transcription level of iNOS was dramatically decreased by DM-0.1 treatment (E). *, p < 0.05 versus the saline group.