Females were held on water only for 24 h prior to infection with P. falciparum via an artificial blood meal. Head, midgut (Mg), and abdominal body wall (Abd) tissues were isolated for qRT-PCR expression analysis of (A) AnsILP2, (B) AnsILP3, (C) AnsILP4, and (D) AnsILP5. AnsILP expression in response to infection is shown relative to expression in matched tissue controls (set at “1”) from mosquitoes fed an uninfected blood meal. For statistical analysis, data were log-transformed and analyzed by ANOVA followed by Newman-Keuls post-test (α = 0.05).