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. 2011 Aug 8;194(3):367–375. doi: 10.1083/jcb.201012132

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© 2011 Couto et al.

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Figure 3. — Adprt1a is required for PARylation after DSBs. (A) Ax2 cells were treated with phleomycin (micrograms per milliliter) and subjected to immunofluorescence using PAR antibodies. Data are representative of three independent experiments. Cells were categorized into those that exhibit pannuclear staining or PAR nuclear foci. (B) Ax2 cells were treated with 1 mM NU1025 or DMSO before exposure to 100 µg/ml phleomycin for 30 min. Cells were subjected to immunofluorescence using PAR antibodies. (C) The indicated strains and their respective parental controls, Ax2 and Ax4, were assessed for PARylation after phleomycin treatment as in B. The percentages of PAR-positive cells were scored from a population of >200 cells. Error bars represent the SEM from three independent experiments. *, P < 0.05 compared with the positive control (Ax2 + phleomycin).