Figure 1.

Effect of L19 on poly(I:C)-induced activation of ISRE promoter and IP-10 production. (A) HEK293-TLR3 cells were transfected with empty vectors or expression vectors for L19 together with ISRE (IRF3 promoter) reporter plasmids. 18 hrs after transfection, cells were treated with poly(I:C) (10µg/ml) for 6 hrs in the presence of 25µM chloroquine (inhibitor of endosome acidification) or no inhibitor. After poly(I:C) treatment luciferase assays were performed. Luciferase activity is normalized to β-galactosidase activity; results are means±s.d. from three separate transfections. (B) A172 cells were transfected with empty vectors or V5-tagged L19 plasmid. 18 hrs after transfection, cells were stimulated with poly(I:C) (25µg/ml) in the presence or absence 25µM chloroquine for 8 or 24 hrs and thensupernatants were measured by ELISA for IP-10 production. Values represent the mean±S.E. of triplicate samples.