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. 2011 May 11;62(12):4191–4201. doi: 10.1093/jxb/err122

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 4. — (A) Changes in AsA levels and (B, C) mRNA levels of the genes involved in AsA biosynthesis (FaGME, FaG1PP, FaGalUR, FaGLDH, and FaMYOX) and recycling (FaMDHAR and FaDHAR) by QRT-PCR. (D) FaGalUR protein levels in the ripe fruits of the parentals (1392 and 232) as well as lines with high (93-30 and 93-34), medium (93-29), and low (93-67 and 93-72) AsA levels in the segregating population. The QRT-PCR values were normalized against the abundance of FaRib413, using the parental 1392 value as the reference (value 1). The bars represent the mean of at least two independent biological samples ±SD. Different letters indicate a significant difference between samples according to the corresponding ANOVA (P <0.05).