Fig. 5.
Influence of double substitution of NPA motifs and the ar/R selectivity filter of AtNIP5;1 on the transport activity for silicic acid (A), arsenite (B), boric acid (C), and water (D). Transport activity was determined for the water-injected negative control (H2O), native AtNIP5;1 (NPS, NPV, AIGR), or S139A-V250A and A117G-I236S (NPA, NPA, GSGR). Data are means ±SD (n=3) for (A) and (B), (n=5–6) for (C) and (D). Different letters above the columns indicate statistically significant differences at P <0.05 by Tukey's test.