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. 2011 Jul 6;62(12):4127–4142. doi: 10.1093/jxb/err175

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 8. — Subcellular localization of barley MIPs as studied through transient (2 d) expression in onion epidermis. Expression was viewed in epidermal peels using an Olympus FV1000 confocal laser scanning microscope. (A) Candidates genes fused to the enhanced yellow fluorescent protein (EYFP); (B) Cytoplasm marker pSAT6-DsRed2-N1 (DsRED, red fluorescence) and pBIN20-plasma membrane-mCherry marker (pm mCherry, red fluorescence); (C) overlay of (A) and (B); (D) magnified parts of cells shown in (C); (E) transmitted light micrographs of cells shown in (A) to (D). Scale bar (A, B, C, E)=50 μm, Scale bar (D)=25 μm.