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. 2011 Jun 15;31(24):8948–8957. doi: 10.1523/JNEUROSCI.1692-11.2011

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Copyright © 2011 the authors 0270-6474/11/318948-10$15.00/0

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Figure 1. — 5-HT accumulation in AIM and RIH neurons requires mod-5/SERT and cat-1/VMAT. Except in b, which shows a representative photomicrograph of 5-HT immunostaining of the HSN neurons, all the other panels show photomicrographs of 5-HT immunostaining of the head region. All the animals shown were adults with anterior to the left. a, In WT animals, seven neurons in four classes (NSM, ADF, RIH, and AIM) showed 5-HT immunoreactivity. c, In mod-5/SERT mutants, only NSM and ADF neurons showed 5-HT immunoreactivity. d, In cat-1/VMAT mutants, 5-HT immunoreactivity in NSM and ADF neurons was significantly reduced and undetectable in AIM and RIH. e, mod-5/SERT mutants bearing the AN::mod-5 transgene restored AIM 5-HT immunoreactivity. f, 5-HT immunoreactivity in ectopic neurons (RIC, RIG, and RIF). The percentages of each neuronal type stained in these strains are summarized in Table 1.