FIGURE 5.

KLRG1⁺NKG2A⁺ expression on CD8 T cells correlates with protective efficacy during a recall response to γHV68. KLRG1⁺NKG2A⁺ and KLRG1⁻NKG2A⁻ CD8 T cells were FACS purified from the spleens of mice 3 mo after γHV68 infection, adoptively transferred into naive congenic animals, and the mice were infected with γHV68 24 h later. The analysis was performed using the spleens of recipient mice on day 16 p.i. A, Representative plots show the frequency of CD90.2⁺ cells among the CD8⁺ T cell population (top panels) and the frequency of γHV68 ORF6_487–495/D^b-specific cells among the CD90.2⁺ CD8⁺ T cell population (bottom panels). The left panels show the KLRG1⁺NKG2A⁺ adoptive transfer group (iNK⁺), and the right panels shows the KLRG1⁻NKG2A⁻ adoptive transfer group (iNK⁻). Numbers represent the percentage of cells in each gate. B, Bar diagrams show the frequency (left panels) and cell numbers (right panels) of donor CD90.2⁺ CD8⁺ T cells (top panels) and those of γHV68 ORF6_487–495/D^b-specific cells among the donor CD90.2⁺ CD8⁺ population (bottom panels). C, Bar diagram displays the number of latently infected cells per 10⁶ splenocytes. Data are the mean of three individual mice per experiment, and error bars represent SD. Similar results were obtained in three independent experiments.