Figure 3.

Phylogenetic tree and schematic structures of human calpain-related molecules. A. Phylogenetic tree of human calpains. Method: The tree was drawn by the neighbor-joining/bootstrap method after aligning all the sequences using MAFFT v6.240 (at http://align.genome.jp/mafft/, strategy: E-INS-i). Atypical calpains are more divergent than classical calpains. The PalB subfamily consists of the strict PalB group, the TRA-3 group, and the CAPN10 group. B. Schematic structures: Black and green letters indicate ubiquitous and tissue/organ-specific calpains, respectively. See also Fig. 4. Symbols: L and XL, N-terminal and extended N-terminal regions of calpastatin. See Fig. 1 legend for other symbols.