| Step | Details |
| O-ring conditioning | 75°C, 2 min |
| Introduce sample | 60°C |
| Denature Sample | 90°C, 5 min |
| Hybridization | 60°C, 16h |
| Wash 1 | 50°C, flow 10s hold 20s |
| Wash 2 | 42°C, flow 10s hold 20s |
| Wash 3 | 42°C, flow 10s hold 20s |
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