Figure 2. Identification of a positive-regulatory element in the 5′-flanking region of PV.1A.

A putative element positively regulating PV.1A transcription was identified between −300 and −262 bp from the transcriptional start site. 2-cell stage embryos were injected at their animal poles with 20 pg of serially deleted PV.1A promoter constructs driving luciferase reporter transcription. Animal caps were dissected at stage 8.5 and incubated until stage 11 in 0.5X MBS for measurement of luciferase activity as described in Materials and Methods. Experiments were repeated three times using independent sample sets. Data are shown as mean ± SD.