FIGURE 4.

Confirmation of pre-mRNA accumulation in an RNase P mutant strain. Total RNA was separated using 1.4% denaturing agarose gels and subjected to Northern blot analysis. Indicated mRNAs were probed with oligos specific to 3′-exon regions. Total RNA is shown for wild-type (wt) and RNase P mutant (ts) strains grown at either 30°C or 37°C. RNA size markers are indicated next to each blot (Materials and Methods). The predicted positions of pre-mRNA and mature mRNA are shown next to the bands on the blots. For Rps29A/B the pre-mRNA cartoon indicates the 5′ UTR intron. Mature mRNA levels are not significantly enriched in the RNase P mutant strain when normalized to internal Scr1 RNA control levels (values range from 0.6-fold to 1.0-fold), but new bands appear at positions predicted to be the indicated pre-mRNA. The presence of 5′ exons, as well as introns and 3′ exons, was confirmed by RT-PCR (data not shown).