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. 2011 Jul 27;31(30):11055–11069. doi: 10.1523/JNEUROSCI.0479-11.2011

Figure 2.

Figure 2.

GABAergic cn interneurons and Purkinje cells are the first cell types marked by Ascl1CreER GIFM. A, Coronal section of the adult cerebellum highlighting where images in B–E and I were taken. B, Double-labeling immunohistochemistry for GABA (green) and βgal (red) shows that cn interneurons were marked when Tm was administered at E10.5. Anti-calbindin (green) and anti-βgal (red) double-labeling immunohistochemistry shows that fate-mapped cells are Purkinje cells when Tm was administered at E10.5 (C), E11.5 (D), and E12.5 (E). F–H, Dorsal views of whole-mount Xgal staining of P21 cerebella when Tm was administered at E10.5 (F), E11.5 (G), and E12.5 (H). I, BrdU was administered 12 h before Tm administration at E10.5, E11.5, or E12.5 and analysis performed at P21. Double-labeling immunohistochemistry for βgal (red) and BrdU (green) shows that Ascl1CreER GIFM-marked Pcs retained BrdU labeling. J, Quantification of the number of double-positive BrdU and βgal Pcs (mean ± SEM; n = 3; unpaired t test). Pcl, Purkinje cell layer. Scale bar: A, 100 μm; B–E, I, 20 μm; F–H, 600 μm.