Table 4.

Real-Time RT-PCR Analysis of NKCC1 and NKCC2

	Log 2 ratio (HZ/PZ)			Ratio (HZ/PZ) fold change
Gene	Day 49	Day 53	Day 58	Day 49	Day 53	Day 58
NKCC1	1.7	−0.175	1.12	3.25	−1.13	2.17
NKCC2	0.305	0.8	0.305	1.24	1.74	1.24

Note: Log₂ HZC/PZC ratios and expression ratio fold changes (HPZ/PZC) from real-time RT-PCR (see “Material and Methods”) of NKCC1 and NKCC2. The results for NKCC1 mimic those obtained in the microarray analysis, with both day 49 and day 58 animals exhibiting greater than twofold changes in the HZC/PZC ratios. The coefficient of determination (r²) for the linear regression between RT-PCR and microarray analysis was 0.986 for NKCC1 and 0.116 for NKCC2.