Table 3.

FOXO1 mRNA Levels and DNA-Binding Activity After Silencing With FOXO1 siRNA

	FOXO1 mRNA	FOXO1 DNA-binding activity
Negative control	1.00 ± 0.00	1.00 ± 0.0
BMP	0.93 ± 0.08	1.0 ± 0.1
BMP + TNF	3.7 ± 0.3*	1.9 ± 0.2*
BMP + TNF + SCR siRNA	3.4 ± 0.1*	1.8 ± 0.2*
BMP + TNF + FOXO1 siRNA	1.0 ± 0.3**	0.7 ± 0.1**

Note: ATDC5 cells were stimulated with BMP-2 and with TNF-α. This was followed by transfection with either FOXO1 siRNA or scrambled siRNA. mRNA then was isolated, and the level FOXO1 mRNA was measured. FOXO1 DNA-binding activity also was measured using ELISA. Data expressed as mean ± SEM.

^*Significant increase with TNF-α stimulation (p < .05).

^**Significant reduction with FOXO1 siRNA (p < .05).