| 10× PCR buffer | 5 µl |
| 10× UPM | 5 µl |
| MBC2 primer | 1 µl |
| dNTP mix | 1 µl |
| cDNA | 7–13 µl |
| AdvanTaq2 | 1 µl |
| Water to total 50 µl (depending on template volume) | |
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