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. 2010 Sep-Oct;3(5):317–324. doi: 10.4161/oxim.3.5.13108

Figure 4.

Figure 4

Cigarette smoke exposure induces binding of endogenous transcription factors to the COX-2/PTGS-2 promoter. ChIP assay of the binding of NF-YA, ΔNp63α or phospho-ΔNp63α and PEA 3 to COX-2/ PTGS-2 promoter using indicated antibodies. A427 lung cancer cells (expressing LKB1 with altered protein kinase ability) were exposed to control medium, 1% SSE and 0.5% MSE for 24 h. PCR was used to amplify regions of the COX-2/PTGS-2 promoter around the PEA 3-binding sites. Negative control (normal rabbit and normal mouse IgGs) was used to confirm the binding specificity (data not shown).