Suppression of EPSCs was abolished by decreasing the postsynaptic chloride driving force. (a) A decrease in the postsynaptic chloride driving force obtained by reducing the intracellular Cl− concentration from 21.5 to 10.5 mM, which adjusted the Cl− reversal potential to close to the resting potential (−65 mV), failed to exert any effect on the LTP induced by 0.6 mM glycine (n=6). (b) In contrast, the same manipulation completely abolished the suppression of EPSCs induced by 1.5 mM glycine (n=6). Further GlyR antagonist strychnine (5 μM) treatment along with a reduced intracellular Cl− concentration, completely switched LTD to LTP with a comparable potentiation magnitude to the potentiation induced by 0.6 mM glycine (n=6). (c) Statistical plotting of data showing differential effects of the manipulation of intracellular Cl− concentrations on persistent changes in EPSCs induced by glycine. **P<0.01, compared between indicated groups. (d) Summary of data displaying effect of Cl− on persistent changes in EPSCs induced by glycine across a range of concentrations (0.05, 0.6, 1.0, 1.5, and 2.0 mM). **P<0.01, compared with all the other groups.