Fig. 4.

Nrf2 and two of its downstream targets during the postnatal development of the hooded seal. (A) Nrf2 mRNA expression, (B) total Nrf2 (crude extract), (C) Nrf2 nuclear content, (D) glutamate-cysteine ligase modulatory subunit (GCLm) protein expression and (E) glutathione S-transferase (GST) activity in neonates, weaned pups and adult hooded seals. Nrf2 relative mRNA levels were calculated by quantitative real-time RT-PCR and compared with GAPDH levels. One unit of GST is defined as the amount of enzyme that synthesizes 1 μmol product min^–1 at 25°C. Data are means ± s.e.m. *, P<0.05.