Table 1. Primers used in this study for screening WDR36 and STI1.
| Amplicon | Forward PCR primer | Reverse PCR primer |
|---|---|---|
| WDR36 | ||
| Exon 1 | ttctgtcggaacctaacgagc | gagttagaggccaaggaggg |
| Exon 2 | agtaagtgtctttcttatgaagg | atacatgttaccttggcttcc |
| Exon 3 | ggacaaggtgatttcctatcc | ttctgaaaatttatcttcctccag |
| Exon 4 | gagcagatgaacatgcctgg | ctctggcatagatgtttacatag |
| Exon 5 | tagattagtatctaagtctgtgg | tgttatttatagacaaccctcca |
| Exon 6 | atcagaatagtgtgtggaagag | aacactatttcctcaggctaac |
| Exon 7–9@ | cttgaatggtaatacttgctgag | ggactcttagttttacccagac |
| Exon 10 | tttaatgaagtagttgcaatctgg | cttatagcagaacctcaatcac |
| Exon 11 | agtggtaataacatctttgttttg | acaagaccagcatgcacctg |
| Exon 12 | cttgttagattggaaacatattgc | aatattatgatgagaaaccttgag |
| Exon 13–15 | tgaaggctagtcccatatatag | atttgactgcatcaactccctg |
| Exon 16 | taaggcagcctgaatgttagtc | gtttcatgacactactacctgc |
| Exon 17 | gtggtgactttctgatcaatgc | agctgtctacattatcaagcag |
| Exon 18,19 | gatggcatctatgacataagtc | gcattgtcagtgctgtcttac |
| Exon 20,21 | ctgtggtattggtcagaagag | atcttaactactgagaacgctg |
| Exon 22,23 | ttggtgtcatcgtttgtactg | ggaggtgcgattttatttcaag |
| STI1 | ||
| Exon 1 | tgcaaaagaccaatccgtgtc | tcacccaagctgtggcctc |
| Exon 2 | tggctccttagggaaggtgtga | actatcagtgtatcaggacagg |
| Exons 3,4 | ctctttggccttcatgtgaatac | accctctgctgtgaggttacag |
| Exon 5 | atgagtggtgcaaaatctagcca | ggtcaatcaaggtcaacagatg |
| Exons 6–8* | gccttcaggagaacttgatag | taaaacagtaagaaacaatggctc |
| Exons 9,10 | cctaatgtgatttttaagcccaa | gtgagacactgtctcaaacatc |
| Exons 11–14# | tgggtgaggcattagagttgga | agagagatgaggccttctctc |
@Additional sequencing primer: aatagaagtgaatgaatactggag.
*Additional sequencing primer: gccatagaatctgctgtgttaaga.
#Additional sequencing primer: cctgtcacctccatttctctc.