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. 2011 Jul 12;11:291. doi: 10.1186/1471-2407-11-291

Table 1.

Polymorphic genes with reference sequence [rs], PCR and sequence primers, products size and annealing conditions

Gene NCBI
dbSNP ID
Primers PCR
product
size (bp)
PCR
Annealing
temp.
XRCC1 Arg194Trp [rs1799782] PCR Forward:
5'-GCCGGGGGCTCTCTTCTT-3'
PCR Reverse biot:
5'-CTCACTCAGGACCCACGTTGT-3'
Pyrosequence:
5'-GGGGCTCTCTTCTTCA-3'
75bp 62°C

GSTA1 [rs3957356] PCR Forward:
5'-GGCTCGACAACTGAATTCCA-3'
PCR Reverse biot:
5'-TGGCTTTTCCCTAACTTGACTCT-3'
Pyrosequence:
5'-GACTCTTCTTTCAGTGGG-3'
101 bp 56°C

Abbreviations: NCBI = National Center for Biotechnology Information, ID = identification.