Table 1.
Gene |
NCBI dbSNP ID |
Primers |
PCR product size (bp) |
PCR Annealing temp. |
---|---|---|---|---|
XRCC1 Arg194Trp | [rs1799782] |
PCR Forward: 5'-GCCGGGGGCTCTCTTCTT-3' PCR Reverse biot: 5'-CTCACTCAGGACCCACGTTGT-3' Pyrosequence: 5'-GGGGCTCTCTTCTTCA-3' |
75bp | 62°C |
GSTA1 | [rs3957356] |
PCR Forward: 5'-GGCTCGACAACTGAATTCCA-3' PCR Reverse biot: 5'-TGGCTTTTCCCTAACTTGACTCT-3' Pyrosequence: 5'-GACTCTTCTTTCAGTGGG-3' |
101 bp | 56°C |
Abbreviations: NCBI = National Center for Biotechnology Information, ID = identification.