Figure 4.
Effect of PL on the growth of human colon cancer cells in nude mice. SW480 colon cancer cells (5 × 106 cells/100 μL) were injected s.c. into the right flank of the nude mice. From the day of inoculation, the mice were administered a daily i.t. injection of (A) PL (100 μg/100 μL of saline) or i.v. injection of (B) PL (25-100 μg/100 μL of saline). Control mice were injected with the same amount of saline until 1 month after tumor cell implantation. Tumor size was measured with a caliper at the indicated times. The greatest dimension of the tumor and the 1 perpendicular to it were measured using the caliper and tumor size was calculated as 0.5 × (width)2 × length = tumor volume.