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. 2011 Aug 10;6(8):e23054. doi: 10.1371/journal.pone.0023054

Figure 6. Inhibition of p38 MAPKα is not necessary for SB202190-induced acidic vacuole formation.

Figure 6

(A) HT29 cells stably transduced with empty vector or vectors expressing wild type p38α or mutant p38α T106M were treated as indicated. Phase contrast images were taken at indicated time points (original magnification 600×) (B) p38α-, p38α T106M- or empty vector-transduced cells were treated with 5 µM SB202190 or DMSO as indicated and anisomycin-induced p38 activity monitored by the phosphorylation of keratin 20, a bonafide substrate of MK2. p38 and Hsc70 expression are shown as controls. (C) Percentage of vacuolated cells after 6 hours of SB202190 treatment were calculated and plotted. (D) Stably transduced cells were treated for indicated times with 5 µM SB202190, stained with AO and analyzed by FACS as described in Figure 2F.