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. 2011 Aug 10;6(8):e23054. doi: 10.1371/journal.pone.0023054

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Menon et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) HT29 cells were treated with BIRB-796 (1 µM) or SB202190 (5 µM) for indicated times and analyzed by Western blotting with the indicated antibodies. Hsc70 and gapdh were used as loading controls. (B) Cells were treated for 12 h with the indicated inhibitors or solvent control, fixed in 95% ethanol and stained with crystal violet (original magnification 600×). (C) Cells were treated with SB202190 or wortmannin and live cell images (original magnification 600×) were acquired at indicated time points. (D) HT29, WM1617 and WM793 maintained in complete growth medium were treated with indicated concentrations of SB202190, PD098059 or BIRB-796 for 90 minutes. Lysates were analyzed for phosphorylation of ERK1/2. Hsc70 was used as loading control.