Fig. 1.

AAI and OTA affect LLC-PK1 cell viability and proliferation. Cells were cultured in the presence of increasing concentrations of AAI and OTA for 24 h. Cell viability, assessed by measurement of LDH release showed that 10 μM AAI (A) and 25 μM OTA (B) are the highest non-toxic concentrations. After 24 h stimulation with non-toxic concentration of both toxins analysis of BrdU incorporation pointed out inhibition of cell proliferation after delivery of 5 and 10 μM AAI as well as after 25 μM OTA (C). Mean of at least 3 experiments performed in duplicates; *p < 0.05 vs control