Fig. 3.

Expression levels of mutants of malin. COS-7 cells were transiently transfected with the wild-type (WT) or indicated mutant GFP-malin-expressing plasmids. Upper panel RT-PCR products of exogenous wild-type and mutants of malin separated on a 1.5% agarose gel. All mRNA transcripts were detected in comparable amounts. Bottom panel detection of wild type and mutants of malin by Western blot with anti-GFP antibody. Equal amounts of proteins were loaded as shown by the α-tubulin control