Figure 1.

Confirmation of (A) PIAS3 overexpression: A549 cells were transfected with a PIAS3 expressing construct. Controls refer to trasnfection with an empy vector not containing the PIAS3. The protein gel blot shows a significant increase in pIas3 expression as well as the FLaG protein. GAPDH was used as a loading control. (B) STAT3 downregulation by siRNa: A549 cells were transfected with siRNA targeting STAT3. protein gel blotting shows a significant decrease in STAT3 expression. Controls refer to transfection with mismatched siRNA. GAPDH was used as a loading control.