Figure 3.

PIAS3 binding to various transcription factors using a transcription factor protein array. EGR1, ETS, GATA1, NR1I2 transcription factors are identified as novel binding partners for PIAS3. ATF and NFκBp65 have previously been described as binding to PIAS3 protein. (A) PIAS3 purified protein obtained by introduction of PIAS3 expression plasmid into E. coli followed by purification and (B) control protein obtained by introduction of an empty plasmid into E. coli followed by protein purification, were both incubated with the TranSignal TF Protein Array membrane for 2 h and detected by PIAS3 monoclonal antibody (Santa Cruz) and anti-mouse Ig HRP conjugate (Amersham). Images were acquired by exposing the membranes using Hyperfilm (TM) ECL.