Figure 7.

PIAS3 has functional effects on EGR1 transcriptional activity. In order to elucidate if PIAS3, in addition to binding, has functional effects on EGR1, A549 cells were transfected with vectors containing PIAS3, EGR1 and a vector containing the luciferase reporter gene under the transcriptional control of EGR1 (EGR1-LUC). After 48 h post-transfection, cells were harvested and relative luciferase activity was measured. The columns denote relative luciferase activity from triplicate experiments. This figure shows that EGR1 transcriptional activity is PIAS3 dose-dependent, with increasing doses of PIAS3 resulting in increased EGR1 binding to its consensus sequence and luciferase activity. The columns with “c” indicate the controls, were instead of PIAS3 expression construct, an empty vector was used.