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. 2011 Aug 10;6(8):e23256. doi: 10.1371/journal.pone.0023256

Figure 2. BTZO-15 increases HO-1 mRNA levels and suppresses NO-induced cell death in IEC-18 cells.

Figure 2

(A) Real-time PCR expression analysis showing the effects of BTZO-15 on the induction of HO-1 mRNA in IEC-18 cells. The cells were treated with the indicated concentrations of BTZO-15 for 24 h. HO-1 mRNA levels were measured by real-time PCR and normalized by GAPDH mRNA. Results shown are the mean ± SD, n = 3. (B) Cell death inhibitory activity of BTZO-15 in IEC-18 cells. The cells were cultured in the presence of either vehicle or the indicated concentrations of BTZO-15 in serum free medium for 1 h. The cells were then treated with 130 µM NOR3 and cultured for 24 h. Cell viability was determined using the WST-8 cell respiratory assay. The experimental value for cell death inhibitory activities is expressed as relative viability and is the mean ± SD, n = 3.