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Three days after adding HGF protein, the enzymatic activities of MMP-2 and MMP-9 were examined by zymography. For the HDFs, MMP-2 activity increased after adding HGF protein except 40 ng, and MMP-9 activity did not changed (A). However, after simultaneously adding TGF-β1, MMP-2 and MMP-9 activity were unchanged (B). In the KFs, (C) MMP-2 activity increased with increasing HGF protein in a dose-dependent manner.
