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. 2000 May 1;105(9):1269–1277. doi: 10.1172/JCI5250

Figure 3.

Figure 3

Effect of adrenalectomy (ADX) and low-dose corticosterone replacement on AP POMC mRNA in WT and CRH KO mice. (a) In situ hybridization of the pituitary with a radiolabeled POMC antisense riboprobe (representative of n = 3 per genotype and treatment group). The neurointermediate lobe is oriented at the left side of each panel. Basal levels of POMC mRNA are equivalent in WT and KO mice, and increase after adrenalectomy in AP corticotrophs. The neurointermediate lobe of the pituitary demonstrated unchanged hybridization intensity before and after adrenalectomy. (b) Quantitative analysis of POMC mRNA hybridization signal intensity in the AP. AP = 0.01 vs. WT Sham and WT ADX+B; BP < 0.05 vs. WT Sham and WT ADX + B.