Figure 1.

The increase of EAPP and p21 upon DNA double strand break induction shows a similar pattern. (A) U2OS cells were treated with 13 µM etoposide and harvested after indicated time points. Protein levels were determined by western analysis. Means and standard deviations of three independent experiments of two series are shown. (B and C) The corresponding p21 protein and mRNA levels were obtained by western [p21 (187)] analysis and RT-PCR. p21 mRNA levels of the high EAPP series were determined only once. The quantification was done with ImageQuant with Actin (AC-74) for western analysis and GAPDH for RT -PCR as references.