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. 2011 Jul 1;10(13):2140–2150. doi: 10.4161/cc.10.13.16227

Figure 1.

Figure 1

Ultrastructural analysis of the skin from wild-type and Cav-1-/- mice. (A and B) Transmission electron microscopy (EM) micrographs of dermal collagen from wild-type (A) and Cav-1-/- (B) mice. (C and D) Distribution of diameters from dermal collagen from wild-type (C) and Cav-1-/- (D) mice. An Image J algorithm was designed to automatically obtain collagen fiber diameters from EM images (Sup. Fig. 1). Using this algorithm, the diameter of 1739 and 2643 collagen fibers was determined from wild-type and Cav-1-/- mice, respectively. Note that dermal collagen from Cav-1-/- mice exhibited a more compact and uniform pattern of fibril diameter and distribution, than wild-type. Indeed the mean and the median collagen diameters from Cav-1-/- mice were significantly smaller than those from wild-type mice (p < 0.001, as determined by Mann-Whitney Rank Sum Test using SigmaPlot). Scale bar = 100 nm.