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. 2011 Jul 1;10(13):2140–2150. doi: 10.4161/cc.10.13.16227

Figure 6.

Figure 6

Inflammatory cell content of the skin in wild-type and Cav-1-/- mice. (A and B) Frozen sections from the skin were stained with the F4/80 antibody, a macrophage cell marker, to evaluate macrophage infiltration in the dermis of wild-type (A) and Cav-1-/- mice (B). Immuno-staining indicates positive labeling of macrophages. (C and D) Histochemical staining to detect mast cells. Using this approach mast cells stain purple. Scale bar = 50 microns. (E) Quantification of mast cells in the skin from wild-type and Cav-1-/- mice. The number of mast cells was determined in five equal areas of skin (n = 5), from the sub-endothelium to the muscle. Note that increased infiltration of macrophages and mast cells was observed in the dermis of Cav-1-/- mice, as compared with normal wild-type dermis. Results are indicated as the mean ± SEM. p values are indicated in the graphs, as determined by the Student's t-test.